Viva voce
bioorganic and medicinal chemistry bsc
Questions on biochemical analysis
Question number 1
- What is chromatography ?
Ans. Chromatography is a technique for the separation of mixture of solutes by distribution of dissolved substance between two immiscible phases.
- What is the stationary phase in paper chromatography ?
Ans . The stationary phase is the liquid held in the fibres of the filter paper.
- What is developer
Ans. developer is the mobile phase which is the solvent or the mixtures of solvents.
- How are the different components of a mixture detected in paper chromatography?
Ans. Components of a mixture are detected by spraying the special spraying Regent on the chromatogram.
- How are the components of a mixture identified ?
Ans. Different components of a mixture are identified by measuring there Rf value.
- What is R_f value ?
Ans. R_f value is the ratio of the distance moved by the component in a mixture to the distance moved by the solvent from the original baseline.
- What is the absorption spectrum ?
Ans. It is the plot of the absorbance of a sample as a function of wavelength.
- What is λmax ?
Ans. Wavelength at which there is maximum absorbance is called λ_{max} .
- What is the calibration curve ?
Ans. It is the plot of absorbance As a function of concentration.
- What are the reducing sugars ?
Ans.Sugars that reduce tollens Reagent or fehling solution are known as reducing sugar. for example glucose.
- What are osazones ?
Ans Carbohydrates with Alpha hydroxy aldehydes ketones like glucose or Fructose reacts with excess of phenylhydrazine to form phenyl hydrazones is called osazones.
- What is a biuret test ?
Ans.Compounds with peptide Bond form a purple Complex With biuret Reagent.
- What is spraying reagent in the separation of amino acids ?
Ans Ninhydrin solution is spraying reagent in the separation of amino acids.
- Which enzyme is present in saliva ?
Ans Amylase enzyme is present in saliva.
- What is zweitter ion ?
Ans Dipolar Ion of amino acids are known as zwitter ion.
- What is saponification value ?
Ans The amount of potassium Hydroxide required to completely hydrolyze of oil or fat is known as saponification value.
- What is iodine value ?
Ans The number of grams of iodine that will combine with hundred gram of the oil or fat is known as iodine value.
- What is the wij’s Reagent ?
Ans Iodine solution in acetic acid is known as wij’s solution.
- Which sugar is present in DNA ?
Ans Deoxyribose is present in DNA.
- What is the use of aspirin ?
Ans Aspirin is used as an analgesic and antipyretic.
- What is acetylation ?
Ans acetylation is a reaction that introduces an acetyl functional group into an organic chemical compound.
- What is the use of Barbituric acid ?
Ans Barbituric acid is used as hypnotic and sedative.
- What is the use of the propranolol ?
Ans propranolol is a Beta blocker and is used to treat hypertension
biochemical analysis viva question with answer
20+ viva question for bsc chemistry
short answer type question for bsc chemistry
biochemical analysis bsc 1st year